Run PCR using a Primer Set

Operation

1. Click the "PCR" button.
   • The registered primer list will be displayed.
2. Select the primer set with mutations or tags to be used for PCR, and click the "Reaction" button.
   • The "Priming Site" window will be displayed, and the number of priming sites for each primer will be displayed on the left.
3. Make sure that the number of priming sites for each primer is 1, and check both.
   • The actual priming sites will be displayed in the list on the right.
4. Click on any line of the priming site.
   • The feature map will jump to that position.
5. Click the "Amplify" button.
   • A confirmation message for "Start PCR" will be displayed.
6. Click "yes(Y)".
   • The "Amplify" list will be displayed. The PCR products are listed.
7. Check them and click the "Load" button.
   • The Directory Chooser will be displayed.
8. Specify the directory to save the file and click "OK".
   • The confirmation message "Completed!!" will be displayed.
   • The icon of the PCR product will be displayed on the Main Tree.
9. Click on the icon of the PCR product.
   • The PCR product will be displayed on the feature map.