In the PCR function of IMC, the priming site of the registered primer set is searched from the genome base sequence. If both primers have a priming site on the genome, PCR amplify the region sandwiched between the primers.
In this case, adenine can be made to protrude by 1 base. It is used for TA cloning.
An annotation is inherited also in the amplified PCR product when the template DNA sequence is annotated (Feature).
It is also possible to perform PCR on multiple template DNA sequences.