Function

• Perform PCR using multiple pairs of primer sets for multiple template DNA fragment sequences, simultaneously generate multiple PCR products, and save PCR product sequence files separately.

Data, software to use

• Multiple template DNA fragment sequence files (supported formats: DDBJ, EMBL, GenBank, FastA)
• Multiple pairs of PCR Primer sets

IMC Edition:

• IMCGE, IMCPE

Overview of operation

1. Launch any edition of IMC.
2. Click the PCR for Multiple Templates button.
3. Select all template DNA fragment sequence files to be PCR-targeted.
4. Select multiple PCR Primer sets.
5. Specify the maximum PCR product length allowed.
6. Click the Reaction button.
7. A list of PCR products will be displayed.
8. If necessary, save as an annotated sequence file.