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IMC is characterized by being able to perform operations of cloning experiment on computer. In this case, special data is unnecessary and it is possible to clone DNA sequence data which can be obtained from a public database such as GenBank or EMBL as it is. For cloning, restriction enzyme digestion, PCR primer design, PCR amplification, and ligation can be performed without changing the annotated sequence. All resulting cloning products are output in GenBank / EMBL format. Since Primer information is pasted and stored on the DNA sequence, it is also useful for Primer management.

It is a cloning function that can actually cut / ligate.

in silico cloning experiment is possible.

It will help you to understand invisible molecular biology experiments. It can be used for assisting and simulating molecular biology experiments.

You can construct arbitrary Vector / Plasmid.

We will list optimal restriction enzymes for Vector insert check and simulate the gel electrophoresis results.

• Restriction enzyme treatment (restriction enzyme map, restriction enzyme digestion fragment generation, optimal restriction enzyme candidate list for insert check, others)
• PCR primer design,
• Primer design to avoid specific features,
• PCR replication (including annotation),
• Batch primer design to amplify all genes,
• Primer management ligation, self-ligation,
• Checking compatibility of base fragment end shape
• Plasmid map creation (Insert area blowing function) (Layout style correspondence)
• Addition of restriction enzyme recognition site to cloning DNA terminus with annotation described
• DNA terminal blunting, phosphorylation (dephosphorylation
• Arbitrary region extraction of annotated base sequence