Register the priming site as a feature.
Perform PCR
- Click the "PCR" button.
- A list of registered primers will be displayed.
- Select the primer set with mutations or tags to be used for PCR, and click the "Reaction" button.
- The "Priming Site" window will be displayed, and the number of priming sites for each primer will be displayed on the left.
Register the priming site as a new feature
- Click the "Insert New Feature" button.
- The "Insert New Feature" confirmation message will be displayed.
- Click "Yes(Y)".
- The "Completed!!" message will be displayed.
- Click "OK".