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On the design of gene composition for recombination into heterologous host
Composition automatic design
On the design of gene level
It is a function to check if the gene cluster is designed correctly.
Gene cluster sequence fragmentation design to reconstruct cluster by OGAB method.
Design homology arms for homologous recombination and PCR primer design for amplification of homology arms.
After designing insert sequences and homology arms, actually perform homologous recombination into the genome.