Genome Analysis Software Info Site
Official Technology Information Site of in silico biology, inc.
Function Menu
License Activation
Function Overviews and Basic Operation
Work Bench
Feature Map- Feature Key and Feature
Management and Operations of Feature Keys- Attributes of Feature Keys
- IMC Original Set Feature Keys
- Types and Roles of Qualifiers
- IMC Original Set Qualifiers
- View and Edit Qualifiers
- Feature Position on Genome Sequence
- Feature Fragmentation
- Feature Synthesis
- Feature Fusion
- Feature Operators
- Link and Refer on Feature
- Feature Mapping
- Register, Edit, Delete Feature
- Feature Appearance
- Join and Delete Position
- Feature Categorization and Presentation
- Import Feature
- Export Feature
- Numbering of Feature
Sequence and Data Input and Output- Genome / Sequence Viewer / Editor
GenBank EMBL Viewer
Sequence Viewer
Annotation Viewer- Multiple Genome Viewer (Linear Map)
Circular Genome Viewer-Designer
Plasmid Map Viewer-Designer
Trace Viewer - Editor- Labeling and Coloring
- Description Window
- Amino Acid Sequence Profile Viewer
- Multiple Alignment Viewer
Phylogenetic Tree Viewer- GT Alignment Viewer
- Restriction Enzyme Map Window
Search Sequence and Annotation- Cloning
- Sequencing
- Gene and Genome Sequence Analysis
- Genome Annotation
Genome Comparison- Multiple Aligment
- Phylogenetic Tree
- Multiple Linear Genome Map
- Gene Cluster Alignment
Multiple Circular Genome Map
Dot Plot Analysis
Venn Diagram Analysis- Core Genome Analysis
- Global Genome Rearrangement Analysis
- Local Genome Rearrangement Analysis
- Mutation Analysis
- Enzyme Alignment by EC Number
- Unique Region Analysis
- Genome Mapping
- Expression Analysis
- Metabolome Analysis
- Genome Design
Settings- Tools
- Window and Dialog Description
- What is "Do It Yourself" Genome Analysis Software?
- Functions and Operations of GenomeTraveler
Dongle License (HW Key)
IMC Primer
Terminate IMC
Toolbox
Feature Key Search
Keyword Search
Pattern Search
Priming Site Search
Batch Homology Search
Restriction Enzyme
Primer Design
PCR Reaction
Ligation
Gel Electrophoresis
Fragment Modification
DNA Content Analysis
Codon Analysis
ORF Analysis
Database Management
Reverse Complement
IMC i02J Primer Desing for In-Fusion PCR
In-Fusion PCR Cloning Primer Design Function
Currently, only the primer design function is installed. PCR cloning using this primer cannot be performed on the IMC.
What is In-Fusion primer design?
In-Fusion PCR cloning is a method that does not require end blunting or ligation, and allows PCR fragments to be cloned into any vector in one reaction. Efficient cloning is possible by adding the terminal sequence of a linearized vector to the primer of the DNA fragment to be inserted.
How to use In-Fusion primer design
- Load any vector sequence into the feature map.
- Click the "In-Fusion" button.
- The "In-Fusion Primer Design Dialog" will be displayed.
- Specify the forward primers you have already created in "Primer 1" and "Primer 2".
- Specify the restriction enzyme recognition site in the MCS of the vector in "Restriction Site".
- Click "Run".
- The designed "In-Fusion" primer set will be displayed.
- Specify the generated primer set and click "Save as a (New) Primer..." to register this set in the "Primer List".
- Select the generated primer set and click "CSV" to save it as a file that can be read in Excel.