Genome Analysis Software Site

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IMC iX01 Several DNA DNA Sequence Digestion, Gel Electrophoresis, Band Excision

Several DNA sequences are selected, restriction enzyme digestion cleavage is performed, and gel electrophoresis is carried out.

Operation

Load multiple base sequence files into the main current directory.

Select multiple base sequences simultaneously.

From the menu, choose Cloning -> Restriction Enzyme -> RE Registration & Editing.

The Enzyme Selection dialog is displayed.

Select one restriction enzyme that digests and cleaves the selected DNA fragment.

Click "Show Recognition Site ...".

The Recognition Site dialog is displayed.

Gel Electrophoresis

Click the Gel Electrophoresis (all) ... button.

The Gel Electrophoresis dialog is displayed.

Band Exicision

Move the mouse over the lane.

As the mouse moves, the band size at that position will be displayed.

Mouse click on one band.

The band is displayed in red.

Right click mouse on red band.

A pop-up menu will be displayed.

Click Load Band (s).

Load Band (s) An execution confirmation message will be displayed.

Click "Yes (Y)".

The base sequence corresponding to that band is loaded into the current main directory.

A completion message is displayed.

Click "OK".

The completion message closes.

Click the loaded band array in the current main directory.

The feature map is displayed.

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