IMC J04E Eukaryotic Gene Prediction by AUGUSTUS

Predicts genes (gene/CDS) from a eukaryotic genome sequence using the external tool AUGUSTUS.

Operation

1. Load the target genome sequence for gene prediction into the current folder. Normally a FastA-format nucleotide sequence file is used, but a GenBank/EMBL-format file that already has some annotation is also acceptable.
2. Confirm that the loaded sequence is displayed as the current genome in the main feature map.
3. From the main menu, select Genome Analysis > ORF Finder > Eukaryotic Gene Prediction by AUGUSTUS.
4. A confirmation dialog for AUGUSTUS is displayed.
5. Select the species option to use as the prediction model. If the model species differs from the species being analyzed, select a closely related species.
6. Clicking "Yes" starts execution, and a progress dialog is displayed while it runs.
7. When the prediction finishes, the dialog closes and the predicted genes are registered and displayed as gene and CDS Features on the current genome.
8. Since this prediction result is not saved automatically, use the Save or Save As function if you want to save the result.