Genome Analysis Software Site

in silico biology, inc.

IMC O13A Operation of Mutation Search

Detects one base mutation between closely related genomes and generates a list of all mutations. In addition, from the list you can display the variation of the reference feature map and the base position of the corresponding main feature map.

Preparation

Load the base stock genome into the current main feature map.

Load multiple closely related genomes to compare with the current reference feature map.

 

Operation

Select Genome Analysis -> Compare -> Mutation Search from the menu. 

The Mutation Search Setting Dialog Box will be displayed.

All genomes loaded in the current reference feature map are listed.

Check the genome (multiple designation possible) to extract the mutation.

Click "Run".

A confirmation message "Start Mutation Search?" Is displayed.

To change the execution parameters, click the Show Parameter button.

Click "Yes (Y)".

Execution is started and a progress message is displayed during execution.

When execution is completed, the list of detected mutations is displayed.

In this list, the mutation points detected in each row are displayed. The mutation point shows the base position by genome and its base call.

When you click on one row (mutation point), the main feature map and the reference feature map are automatically shifted, and the mutation part is displayed as the center of the map.

Mutable bases are highlighted on the sequence lane of the main feature map.

On the other hand, in the reference feature map, the variant base is displayed at the mutation site.

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