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GT D5A Data Format Conversion from AFG to BAM

Velvet assembler output file Convert AFG format file to BAM format file.

There are two ways.

First, if there is an AFG file loaded in the Analysis node of GT's TREE pane, you can perform conversion directly from that node.

The other is executed from GT's Tools menu.

This section explains how to perform conversion from an Analysis node.

Implementation Edition

GenomeTraveler

Operation

  1. This is explained using training data.
  2. Double-click the Analysis node in the Velvet assembly.
  3. The afg file node is displayed.
  4. Right-click on the afg file node on the mouse.
  5. The menu will be displayed.
  6. Select "Convert Afg to Bam".
  7. A confirmation message will be displayed.
  8. Click "Yes (Y)".
  9. The conversion is executed.
  10. A progress message is displayed during execution.
  11. When execution is completed, a completion message is displayed.
  12. Click "OK".
  13. The completion message closes.
  14. The node file has been converted to BAM.
  15. Right-click on the BAM file node on the mouse.
  16. The menu will be displayed.
  17. Launch Alignment Viewer "Show Read Alignment" and display BAM information "Show BAM Information" are possible.

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