Genome Analysis Software Info Site

IMC has various PCR Primer design methods.

The ability to simply drag the nucleotide sequence on the sequence lane and register it as a primer

• Register primers from the sequence lane
• Register primers from the feature lane

Ability to design features on the feature lane and primers to amplify inside and outside the selected area

• Primer design for amplifying included products, including selected features of feature lanes
• Primer design for amplifying the product containing or containing the selected genomic region of the feature lane

A function of designing primers that amplify a large number of areas at once (with Iterate Design function: it is a function to repeat the design until there is no area that can not be designed)

• Batch PCR Primer Design Batch PCR primer design to amplify all features with specified feature key on genome
• Whole Genome Covering PCR Primer Design PCR primer design that PCR product completely overlaps whole genome or large genomic region with PCR product overlapping
• Sequencing Primer Design: Sequence primer design for sequencing DNA fragments that can not be covered with one lead of a capillary sequencer
  reference

The following is a function to design a group of primers for cloning multiple DNA fragments at once, such as gene cluster design. From design to cloning, you can load cloned products into the IMC.

• In-Fusion Design Ver.1: Primer design function for In-Fusion Cloning
• In-Fusion Design & Build Ver.2: Primer design function for In-Fusion Cloning
• In-Fusion Design & Build Ver. 3: Ability to design and build gene clusters by dropping DNA fragments to be inserted into vector sequences
• Batch Gene Cluster Design & Build: the ability to design and build lumped numerous gene clusters by replacing the gene sets that make up the cluster
• Combinatorial Design & Build: The ability to combinatorially combine design and build a fragment constituting a gene cluster