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IMC i02P1 Add Restriction Enzyme Recognition Sequence to End of Primer sequence
Apply a specified restriction enzyme recognition sequence at the 5 'end of the registered primer sequence.
Operation
Edit the primer for which restriction enzyme recognition sequence is to be added.
Click "Primer Registration" from the menu.
The "Primer List" dialog will be displayed.
Check to the primer to be edited.
Add a restriction enzyme recognition sequence to the 5 'end of the primer.
Click the "Add Enzyme Site" button.
The "Enzyme Selection" dialog is displayed.
Select one restriction enzyme and click the "Add Enzyme Site" button.
The "Add Enzyme Site?" confirmation message is displayed.
Click "Yes".
A restriction enzyme recognition sequence is appended with lower case letters at the end of the Primer to be edited in "Primer List".
Primer sequences written in lowercase letters are not subject to priming complementarity check.