Ligate the ends of one DNA sequence.
This is called self-ligation.
When self-ligation is performed in IMC, its DNA sequence attribute is changed from Linear to Circular.
Therefore, if you display that feature map, you can scroll horizontally indefinitely in the same direction if you could only scroll to the end until then.
In order to return this to the original linear DNA, it is necessary to cut at the same position.
If both ends are cleaved with the same restriction enzyme, digestion with the restriction enzyme cleaves it back to the original linear DNA.