Ligation of two different DNA sequences to generate one DNA sequence.
Operation
Load two DNA sequences into the main current directory.
Here we use the sample DNA fragment Bsub_50 kb.gbk sequence as a sample of DNA fragment sequence digested and digested with a specific restriction enzyme (BamHI) in advance.
Since these fragments digested and cleaved with one restriction enzyme, they have the same terminal shape.
From the menu, choose Cloning -> Ligation -> Simple Ligation.
It is checked in the array of all the libraries on the list.
Click "Load ...".
Loading is executed immediately and a completion message is displayed when it is completed.
At the same time an array of all the selected ligation products is loaded into the main current directory.
It is well understood that the relationship between the four generated ligation products is copied to the reference feature map and viewed in parallel.
Right click on the reference directory to copy. A pop-up menu will be displayed.
Select "Change Current Directory".
A confirmation message "Change Current Directory?" Is displayed.
Click "Yes (Y)".
The copy destination is the current reference directory.
Return to the main directory and select all four generated sequences.
Then click the mouse.
A pop-up menu will be displayed.
Select "Copy to Ref".
All generated ligation product sequences are copied to the current reference directory.
Let's look at the four ligation product sequences on the reference feature map.
It turns out that the arrangement of the first lane and the fourth lane and the arrangement of the second lane and the fourth lane are reverse complementary sequence to each other.