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IMC W441 Ligation Dialog Description

In the Ligation dialog, a list of the DNA sequences loaded in the main current directory is displayed and ligation of the selected sequence is executed.

1st radio button: When on, we make that sequence the first fragment.

2nd radio button: When on, sets the sequence as the second fragment.

DNA shape field:  is displayed when the array is circular,  is displayed in the case of Linear.

Name field: The file name of the DNA sequence file is displayed.

Length field: The sequence length of the DNA base sequence is displayed.

5 'End Type field: The 5' end shape of the DNA base sequence is displayed. If phosphorylated, PPP- is added. The bases displayed in green indicate base pairs that do not exist. That is, it indicates that the base shown in black protrudes with a single strand.

3 'End Type field: The 3' end shape of the DNA base sequence is displayed. If phosphorylated, PPP- is added. The bases displayed in green indicate base pairs that do not exist. That is, it indicates that the base shown in black protrudes with a single strand.

Self Ligation check box: If checked, the ligation to be executed will be self-ligation. The 2nd radio button column becomes Inactive. When unchecked, it becomes a ligation reaction between two sequences. 2nd radio button becomes active.

End Check checkbox: If checked, ligation will be performed if the terminal shape is complementary and phosphorylated. Ends that do not fit are not subject to ligation.

Ligation execution button: When this button is clicked, ligation judgment between the selected DNA sequences is executed.

Close button: Click this button to close the dialog without executing ligation.

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