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in silico biology, inc.

IMC E04T01 Create Local Blast Database from Amino Acid Sequence File

  1. Let's create a network sequence database for executing Blast search on local PC or Mac from amino acid sequence file.
  2. As an example, we will create a Blast database so that the COG amino acid data file can be searched for in Blast.
  3. The COG amino acid data file was copied when IMC was installed, and it is saved below.
  4. On Windows, it is stored as myva in c: \ Program Files (x86) \ isb \ imc \ data.
  5. Copy this file to a temporary storage place in advance. For example, create a temporary directory such as user \ Work and copy it there.
  6. Operation Start IMC and select File -> Create Blast DB ... from the menu.
  7.  
  8. The "Blast DB List" dialog is displayed.
  9.  Click "Add Amino Acid DB ...".
  10. The "Blast DB Setting" dialog is displayed.
  11. Amino Acid File (S): Click "Ref ..." on the right side of the column and select the COG file myva that you previously copied.
  12. DB Name: Enter any database name directly in the input field. For example, enter "COG".
  13. In this time, in order to create a database locally, I have a writable directory of PC or Mac currently in use.
  14. By default, it is generated in the following directory. In this time, in order to create a database locally, I have a writable directory of PC or Mac currently in use.
  15. By default, it is generated in the following directory.
  16. C: \ Users \ user \ imc_xx \ data \ db
  17. Click "Run".
  18. A confirmation message "Create Amino Acid DB?" Is displayed.
  19. Click "Yes (Y)". Click "Yes (Y)".
  20. Execution of the database generation starts, and a progress message is displayed during execution.
  21. When generation is completed, a "Completed" message is displayed.
  22. Clicking "OK" will close the "Completed" message and display the database generated in the Blast DB List.
  23. A database for local blast search has been generated.

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