Genome Analysis Software Site

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IMC W212C Semi Auto Annotation Dialog from Reference Genome

Auto Annotation by Reference Data result dialog for operation.

  • Add annotations semi-automatically to the main current genome sequence with the reference genome as the database for Blast search.
  • With this annotation function you can annotate multiple genome sequences at once, so the Name field will display a list of annotated main current genome sequences.
  • If you click on one of them, the results belonging to that array are displayed.

The Screening field is for narrowing down search results. Three key types are as follows.

  • Identity: The degree of agreement (percentage) in the alignment region between the query sequence and the hit sequence
  • E-Value: The probability that a sequence that coincidentally coincides in this search space when compared with a random sequence of the same composition
  • Bit Score: Search Sum of match / mismatch score of query array and hit array specified in matrix to be used Screen: Enter the range of each value and click this button to narrow down.

Hit list: Hit subjects are listed for each feature of the reference genome as a query sequence.

Explanation by column

  • Check box: Selected when checked Feature Key: Feature key of the query feature on the reference genome
  • Query Length: Base length of the feature to be a query on the reference genome
  • Subject Length: Base length of reference genome sequence
  • Identity: The degree of agreement (percentage) in the alignment region between the query sequence and the hit sequence
  • Alignment Length: Base length of alignment region
  • Mismatches: Mismatching number of bases between query sequence and hit subject sequence in alignment region
  • Gap Openings: the base length of the generated gap in the alignment region
  • Query Start: Relative starting base position of the query feature sequence
  • Query End: Relative end base position of query feature sequence
  • Subject Start: Absolute starting base position of reference genome sequence
  • Subject End: absolute end base position of reference genome sequence
  • E-Value: The probability that a sequence that coincidentally coincides in this search space when compared with a random sequence of the same composition
  • Bit Score: Search Sum of match / mismatch score of query array and hit array specified in matrix to be used
  • Sequence: Array of alignment regions of hit subjects
  • Alignment (Figure): An illustration of the total length and alignment region of the query sequence and the hit subject, respectively
  • Alignment (Button): When clicked, a pairwise alignment dialog of query array and hit subject array is displayed.
  • Gpff (Button): When clicked, the Feature Key, Qualifier, Value list of the GPFF of the hit subject is displayed, and the selected entry can be copied as Qualifier.

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