Genome Analysis Software Site

in silico biology, inc.

IMC i01N Register New Restriction Enzyme

As an example, register a restriction enzyme (BspQI) with the following recognition sequence and cleavage site.

5'-GCTCTTCn^nn-3 '

3'-CGAGAAGnnnn^ -5 '

The cutting place is indicated by "^".

Operation

  1. From the menu, select "Cloning -> Restriction Enzyme -> RE Registration & Editing".
  2. The Enzyme Selection dialog is displayed.
  3. Click "Add ..." at the bottom of the dialog.
  4. The "Enzyme Registration" dialog is displayed.
  5. In the Name text field, enter the restriction enzyme name "BspQI".
  6. Recognition Site 5 'Enter "GCTCTTCn^nnn" in the text field.
  7. Enter "^" at the disconnection point.
  8. 3 'Enter' CGAGAAGnnnn^ 'in the text field.
  9. Click "Save".
  10. The Enzyme Registration dialog closes.
  11. The restriction enzyme "BspQI" is displayed in the Enzyme List.
  12. Hereafter, this restriction enzyme can be used for restriction enzyme treatment.
  13. If you register by mistake, you can edit or delete it.

 

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